The deal will give bigbasket more muscle to compete against rivals like Grofers and e-tailing giant Amazon.
Other investors, who participated in the round include Abraaj Capital, Sands Capital and IFC. The company plans to use the investment to build farmer networks and expand deeper into existing cities.
bigbasket has also renewed its contract with actor Shahrukh Khan to continue as the company's brand ambassador, it said in a statement.
"bigbasket is delighted to receive this funding from Alibaba, which will essentially be directed towards growth and consolidation. The multinational e-commerce retailer fits in best with what we believe in," bigbasket co-founder and CEO Hari Menon said.
More From This Section
"bigbasket is currently partnering with about 1,800 farmers throughout the country and aims to become the 'most preferred partner' for up to 3,000 of them," Menon said.
The company said it is planning to "dig deeper" into the cities it already has foothold in, rather than focus on expansion into more cities.
The deal pegs bigbasket's valuation at over USD 900 million post the transaction.
bigbasket has recently crossed the 8 million customers mark. It closed the previous fiscal year (2016-17) with Rs 1,410 crore in revenue and has broken-even in Bengaluru and Hyderabad.
It expects this number to increase to Rs 300 crore in August, and touch Rs 500 crore in March 2019.
In November last year, Alibaba -- which is also an investor in Paytm -- had sought approval of the Competition Commission of India (CCI) for acquiring stake in bigbasket.
Alibaba is aggressively investing in the Indian market. In 2015, it had invested in e-commerce major Snapdeal.
Its affiliate, Ant Small and Micro Financial Services Group (Ant) has recently pumped in about USD 200 million in online restaurant guide and food ordering app, Zomato.
With people becoming comfortable buying even milk and bread online, the online grocery segment is projected to witness a strong growth over the next few years in India.
According to a report by Franchise India, the online grocery market is expected to be Rs 2.7 billion market by 2018-19.