The 'Saving Mr Banks' actor will play a character who would later become the Ultimate Spider-Slayer, reported Ace Showbiz.
The first generation of spider-slayers was created by his father, Spencer Smythe. Alistair is seeking revenge for his father's death.
The sequel is due to hit theatres on May 2. Andrew Garfield is back as Peter Parker, and Emma Stone reprises her role as Gwen Stacy. Jamie Foxx stars as Electro, Dane DeHaan as Harry Osborn, Chris Cooper as Norman Osborn, and Paul Giamatti as The Rhino.