Leader of Opposition Vijender Gupta said that it was "unprecedented" that a member trying to raise some issues was assaulted by his party colleagues in the House.
"It is a highly condemnable and inhuman act indulged in by the ruling party legislators. The ruling party is using its brute majority to coerce any form of opposition in the House," Gupta said.
The AAP dominates the Assembly. The opposition comprises four MLAs of the BJP-Akali Dal alliance in the 70-member House.
Gupta also questioned the role of Speaker Ram Niwas Goel during the episode that unfolded after Mishra showed a poster in the House, levelling charges of corruption against Chief Minister Arvind Kejriwal and Health minister Satyendar Jain.
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"The Speaker expressed his unhappiness over the attack on Mishra, but took no action against the AAP MLAs who attacked him. This will only embolden them to resort to such acts in the House," he said.
"Manhandling of Mishra clearly shows that Kejriwal and his party are not open to any dissent or opposition."
Opposition MLA Manjinder Singh Sirsa demanded action against the AAP MLAs involved in the incident.