All five BJP MLAs -- G Kishan Reddy, K Laxman, NVSS Prabhakar, Ch Ramachandra Reddy and T Raja Singh -- were suspended from the Assembly during the special session today.
BJP had opposed the bill, calling it anti-Constitutional as it sought to increase a religion-based quota.
Wearing black scarves, the MLAs walked to the Assembly from the Ambedkar statue near Hussain Sagar lake before the House proceedings began today.
They shouted slogans in support of increasing quotas for Scheduled Tribes (also provided in the bill) but opposing reservations for Muslims.
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BJP workers who had planned to march to the Assembly were detained in various parts of the city, he claimed.
In Karimnagar, about a hundred BJP protesters were detained by the police.
Under the bill, passed by both houses of the state legislature, quota for STs will be increased to 10 per cent from the existing 6 per cent, while that for BC-E category (backward sections of Muslims) will go up to 12 per cent from the existing 4 per cent.