The Bangladesh Nationalist Party-led 20 party alliance has been protesting and staging shutdowns since January 6 to mark the first anniversary of the controversial polls the BNP's arch-rival Awami League won.
The 20-party alliance demands fresh elections under a non-party caretaker administration.
The 72-hour shutdown will be enforced on top of its nonstop blockade underway since January 6. The fresh shutdown has been announced hours after the five-day 'hartal' by the alliance ended this morning.
The alliance will also take out "mass processions" across the country on Sunday, the DailyStar reported.
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The shutdown is meant to protest "killing of opposition leaders and activists in the name of crossfire, repression, and filing of false cases against alliance leaders," and demanding release of all political prisoners, the report said.
The turbulence in Bangladesh has forced authorities to move the dates of the Secondary School Certificate (SSC) and its equivalent examinations on weekends.
BNP, led by Hasina's arch rival and former premier Khaleda Zia, boycotted the 2014 general election and is enforcing the current violent blockade across Bangladesh, demanding fresh polls.
The prime minister has already ruled out the possibility of talks with the agitating BNP-led coalition to end the political deadlock.