Second seeds Bopanna and Romanian Florin Mergea lost to the unseeded Canadian-Czech pairing of Daniel Nestor and Radek Stepanek 6-3, 6-7(6), 4-10 in an hour and 24 minutes on Court No.3 at the Aviation Club Tennis Centre.
Yuki Bhambri and Mahesh Bhupathi had lost their respective opening matches yesterday.
The Indo-Romanian pair dominated the first set, winning 29 of the 49 points played. Though the unseeded combine earned three breakpoint chances, they wasted them all.