Combining with Edouard Roger-Vasselin of France, Bopanna lost to Oliver Marach of Austria and Croatia's Mate Pavic 6-4 7-6 (7-5) 3-6 at the Melbourne Park.
The match lasted a little over two hours and saw the Indo-French duo convert one out of two break points, while their opponents converted three out of 12 break points.
Bopanna and Roger-Vasselin had two aces compared to their opponents' seven.
In an earlier match, the duo of Sharan and Rajeev Ram from the USA suffered a 6-3 7-6 (7-4) 6-4 defeat at the hands of Lukasz Kubot of Poland and Brazil's world number one Marcelo Melo.
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