Exports can only restart when guarantees of adequate treatment are provided, Judge Djalma Moreira Gomes in Sao Paulo wrote in his decision yesterday, published in the local media.
The injunction was in response to a case brought by an animal rights group, National Forum for the Defense and Protection of Animals.
The forum's civil suit cited conditions aboard the ship "Nada," currently docked in Sao Paulo's Porto de Santos with about 27,000 heads of cattle owned by Minerva Foods and bound for Turkey.
Gomes wrote he was accepting the petition "to prevent the export for slaughter of live animals, from throughout the national territory, until the destination country adopts slaughter practices in line with those of the Brazilian legal system."
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Animals currently aboard the "Nada" must be taken off, he said.
"It can only continue its voyage after the complete disembarkation of the live animals."
The same judge had issued an initial court order on Thursday to stop the ship from leaving port.
The animal rights group bringing the case said it had received "huge support" from other activists.
"We hope that the export of live animals will be definitively banned.