Egyptian Interior Minister Magdy Abdul Ghaffar said that Muslim Brotherhood collaborated with Hamas to kill former general prosecutor Hisham Barakat in a car bomb last June.
Barakatm, 65, died hours after the powerful bomb hit his convoy in Cairo on June 29 in east Cairo.
"This plot was carried out on the orders of the Muslim Brotherhood... In close coordination with Hamas, which played a very important role in the assassination of the chief prosecutor from start to finish," Ghaffar told reporters.
Fourteen Brotherhood members took part in killing Barakat and a group of 48 members planned for the plot, and they were arrested, Ghaffar said.
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"Hamas played a very big role in last year's assassination of Egyptian chief prosecutor Hesham Barakat. Elements from Hamas took part through planning and training," he added.
Barakat, who oversaw cases against thousands of Islamists, was killed in the blast as he left his home. It was the first assassination of a senior Egyptian official in 25 years.
The Brotherhood, Egypt's main opposition movement for decades, was blacklisted as a "terrorist group" in December 2013.