This will mark the Grammy-winning superstar's fifth Grammy performance and his first in four years.
He is also nominated in this year's album of the year category for his work on Adele's "25", reported Billboard.
Mars joins previously announced performers Adele, John Legend, Metallica, Carrie Underwood and Keith Urban.
The Grammy Awards will take place February 12 at the Staples Center in Los Angeles with host James Corden.
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