The assault happened when the 43-year-old rapper was working out at Steel Gym, reported Ace Showbiz.
Rhymes threw the protein drink bottle when he had a heated argument with the worker at the gym. According to police officers, the bottle hit the worker in the back of the head but the worker was not seriously injured.
An eyewitness said that a front desk employee got into a argument with Rhymes on Tuesday because the employee refused to allow cameraman who was going to record the rapper's workout routine inside.
"Things got heated. Busta splashed water on him and the employee threw water back. Busta's security got between them," a bodybuilder from Brooklyn said.
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"The cops came and (the employee) kept changing his story. He was provoked. Busta never had any physical contact with him whatsoever."
Rhymes was soon released from the jail early Thursday. He commented on the incident, "It's stupid." Following his release, the "Calm Down" rapper took to Instagram to tell his fans that he's fine and would go back to work.