"As a security precaution, and to ensure the protection of all staff, our mission in Kiev will be closed until further notice," foreign ministry spokesman Adam Hodge told AFP.
A day earlier, anti-government protesters entered the embassy's reception area seeking refuge.
Canada's foreign ministry had said: "We expect these individuals to be able to leave the embassy safely, free from intimidation, arrest or further harassment."
But officials would not say today if the group was still being sheltered or had left.
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Before the protests, Ottawa had been seeking closer ties with Kiev. Yesterday, Foreign Affairs Minister John Baird announced medical supplies would be provided for the protesters.
A crackdown by Ukraine security forces yesterday triggered a storm of international condemnation, including talk of sanctions.
Overnight bloodshed came after some three months of mostly peaceful protests, which broke out after President Viktor Yanukovych ditched a pact with the European Union in favor of closer ties with former Soviet master Russia.