CBI sources said BSP supremo Mayawati who was at the helm of affairs in UP during 2007-12 during which NRHM misappropriation is alleged, will be called for questioning in connection with the case.
She will be examined soon to unearth the larger conspiracy of the Rs 10,000 scam, the sources said.
The sources said the agency needs clarification on funding of some projects under NRHM in which some top officials including the then Principal Secretary, Health, Pradeep Shukla has been charged by the agency.
As many as five persons had died or were allegedly murdered in an attempt to cover up the large-scale irregularities in the case.
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Babu Singh Kushwaha, a former UP cabinet minister during the Bahujan Samaj Party rule, was arrested on March 3, 2012 and is still languishing in jail.
Kushwaha has recently moved the apex court seeking bail, which was dismissed on February 13, with the court saying the allegations against him were very "serious" and it was a "good case for dismissal".