Monae gave a heartfelt speech in solidarity with female artistes as she introduced Kesha's comeback performance at the 60th annual music gala, which was charged with the Time's Up movement message.
"Artistes, writers, assistants, publicists, CEOs, producers, engineers and women from all sectors of the business. We are also daughters, wives, mothers, sisters and human beings. We come in peace, but we mean business.
The singer also said just as women have the capacity to shape culture, they also have the power to undo a culture that does a disservice to them.
Emphasising on gender inclusion, Monae called on both women and men to build a "united music industry committed to creating more safe work environments, equal pay and access for all women".
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The "Tik Tok" singer was joined by Bebe Rexha, Cyndi Lauper, Camila Cabello, Julia Michaels and Andra Day - all of them dressed in white.
Alessia Cara emerged as the only female artiste to bag a Grammy (Best New Artiste), as Bruno Mars bagged seven awards, followed closely by Kendrick Lamar with five.
Rihanna shared a Grammy with Lamar for "Loyalty", which won the award for Best Rap/Sung Collaboration.
At the red carpet of the Grammys, Lana Del Rey said that the Time's Up movement is here to stay.
"It's not a passing phase. The ball's going to keep rolling (and the music industry is here for it)," she said.
Elton John played the piano which had a white rose on the top, as he performed "Tiny Dancer" with Miley Cyrus in support of the campaign.
Pink, Heidi Klum, Imagine Dragons, Zayn Malik, Rita Ora, Khalid, Sam Smith, Anna Kendrick, Rapsody, Sarah Silverman, Kelly Clarkson, Grammy host James Corden, among others also flaunted white rose on the red carpet.