The agency had arrested Gazdar on February 16.
The 250-page charge sheet contains statements of five persons including Gazdar himself, Zakir Naik's sister and an accountant of Naik's NGO, Islamic Research Foundation (IRF), an ED official said.
The matter is likely to come up for further hearing before the special court for Prevention of Money Laundering Act cases on Monday.
Two days ago, the court had issued a non-bailable warrant against Naik who is believed to be in UAE.
The NIA had filed the case against Naik and some IRF officials under section 153-A of IPC (promoting enmity between different groups on grounds of religion) and the UAPA.
Naik left the country last year, allegedly to evade arrest after perpetrators of a terror attack in Dhaka said that they were inspired by his teachings.