"This is a legitimate call to arms. My daughter Sam was bullied out of school and then called a liar. It's on!," he posted on Twitter.
Sheen asked his thousands of followers to throw rotten eggs and other such garbage in the school premises.
However, Sam's mother Denise Richards, 42, has yet to speak out on the alleged incident.
Earlier this week, she was busy planning Sam's 9th birthday party.