The 'Chelsea Lately' host had rekindled her romance with the hotelier in February last year but the couple has now decided to go its separate ways, reported Us magazine.
"He's been telling his friends they're done. They're definitely over," a source said.
The news comes just six months after the funnywoman complained about her long-distance relationship during a TV interview on Oprah's Next Chapter.
"I have met my match. We couldn't be more opposite. I bring out the stupidity in him probably. We were talking about living our lives together, my boyfriend and I. We've broken up a couple of times and gotten back together, and we're trying to figure out how to live a life together," Handler had said.