"Rather than being innocuous and gentler alternatives to typical horror or drama films, children's animated films are, in fact, hotbeds of murder and mayhem," said the study authors from Canada and UK in the journal BMJ.
On-screen death and violence can be particularly traumatic for young children, and the impact can be intense and long lasting.
Because of this many parents will not let their children see the "endemic gore and carnage" typical of films aimed at adult audiences, researchers said.
They also looked at whether the first on-screen death was a murder or involved a main character's parent.
More From This Section
The study found that two thirds of the cartoons depicted the death of an important character compared with half of the adult films.
Parents of main characters were more than five times as likely to die in children's cartoons as they were in films targeted at adults.
Furthermore, the data suggest that parents, nemeses and children were more often the first casualties in cartoons whereas the main protagonist was the most likely to be killed off in films for adults.
Only cartoons in which the main characters were either human or animal were included in the analysis, as it's not clear if the concept of death among 'humanised' objects, such as cars and toys, exists.
Film genres included 'horror' such as The Exorcism of Emily Rose and What Lies Beneath, and thrillers, such as Pulp Fiction, The Departed and Black Swan.
Grisly deaths in cartoons were common: shootings in Bambi, Peter Pan, and Pocahontas; stabbings in Sleeping Beauty and The Little Mermaid; and animal attacks in A Bug's Life, The Croods, How to Train Your Dragon, Finding Nemo and Tarzan.