The Ministry of Commerce complained US regulators discriminated against Chinese suppliers by using incorrect standards for deciding what production cost and market prices should have been.
Beijing faces mounting criticism from the United States and Europe that it is exporting steel at unfairly low prices to clear a backlog in its glutted home market. Western governments say that hurts their producers and threatens thousands of jobs.
The US Commerce Department announced the penalties on Wednesday to offset what it said was improper subsidies to Chinese steel mills and unfairly low export prices. It imposed similar penalties on steel imports from India, Italy, South Korea and Taiwan.
It said Beijing will "take all necessary measures to fight for fair treatment" but gave no details.
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The latest penalties apply to corrosion-resistant steel products. They include anti-dumping duties of up to 210 per cent and anti-subsidy duties of up to 241 per cent.
On May 17, Washington imposed similar duties of up to 522 per cent in a separate action targeting Chinese-made cold-rolled steel used in automobiles and other manufactured goods.
Chinese government plans call for stepping up exports and shifting some operations abroad. The Cabinet approved measures in April to support steel exports with tax rebates and bank loans.
The European Union launched its own investigation of Chinese steel exports in early May following protests by steelworkers.
In Britain, Tata Steel cited low-cost Chinese competition when it announced plans last month to sell money-losing operations that employ 20,000 people.