The teleoperation centre was established at the Beijing Aerospace Control Centre (BACC), hours after the Chang'e-3 probe soft-landed on the lunar surface.
The moon rover 'Yutu' or Jade Rabbit separated from the lander early morning today.
The successful separation and soft-landing marked the formal operation of the centre, where technicians can fully control and monitor the working conditions of the rover and lander, state-run Xinhua new agency reported.
The centre provides 40 seats for operating posts ranging from commanding to route planning.
Chang'e-3 landed on the moon's Sinus Iridum yesterday, establishing China as the third country in the world capable of carrying out such a rover mission after the United States and former Soviet Union.
Zhou said that commands for future deep space explorations will also be issued from the centre.