Shipments of eligible US beef have been allowed to enter China since yesterday, the General Adminstration of Quality Supervision, Inspection and Quarantine said in a statement.
Announced last month, the lifting of the beef embargo was one of the first concrete results of trade discussions that began when Chinese President Xi Jinping met with Donald Trump at the US leader's resort in Florida in April.
The agreement, seen as a sign of warming trade ties between the world's largest economies, also gives US natural gas and certain financial services access to China's market of nearly 1.4 billion people.
China imposed a ban on US beef imports following a case of mad cow disease in the United States in 2003.
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To ensure the safety and quality of the imported meat, China insists that US beef comes from cattle younger than 30 months.
The animals must be traceable to their birth farm.
"Cattle should be born and raised in the US, or born in Mexico or Canada and slaughtered in the US," China's quarantine authorities said.
"Cattle should not be the offspring of those that were suspected of having or confirmed to have mad cow disease."