Segway, founded by the inventor of the quirky vehicles, last year sought US government action against Ninebot and several other Chinese manufacturers, alleging "violation" of its patents.
Ninebot said on a verified social media account that it had purchased "100%" of the New Hampshire-based firm, without stating how much the deal was worth.
"We believe this acquisition is very meaningful for a new generation of Chinese innovation to spread out to the world, and for China to move from manufacturing to invention," it said on China's Twitter-like Sina Weibo.
It was later acquired by a group called "Summit Strategic Investments", Segway says on its website.
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The Segway was introduced in 2002 amid great fanfare as way to revolutionise urban transport. They use gyroscopes, computers and electric motors to cruise at up to 19 kilometres per hour.
Such machines failed to become a mainstream method of transport but have achieved niche success in China where riders can be spotted in upscale neighbourhoods -- as well as police using them to patrol on Tiananmen Square.
Ninebot also announced Wednesday that it had received an USD 80 million investment from Chinese smartphone manufacturer Xiaomi.