The yuan was fixed at 6.4085 to the greenback, according to the China Foreign Exchange Trade System, just 0.07 per cent weaker from the previous day. But it was the lowest since August 2011, the Shanghai Securities News said on its website.
The yuan still ended the day at 6.4053 to the dollar, up from both yesterday's close of 6.4095 and the reference rate, also known as central parity.
The lower fixing followed an interest rate cut announced on Tuesday.
The yuan is restricted to trading up or down two per cent from the daily rate on the national foreign exchange market.
Before the August 11 change, the central bank used to decide its daily yuan reference rate by polling market-makers, but now says it takes into consideration the previous day's close, foreign exchange supply and demand, and the rates of major currencies.