The advertisement by the political action committee 'Rebuilding America Now' features Clinton, then a Senator, at a conclave hosted by an Indian media house in New Delhi in 2005.
The ad plays her remarks on outsourcing in which she says: "I don't think you can effectively restrict outsourcing, there is no way to legislate against reality so I think that the outsourcing will continue; but I don't think there is any way to legislate against outsourcing. I think that is just a dead end."
The advertisement ends with the messages -- "Outsourcing jobs for dollars" and "She's earned India's trust."
A report in the New York Post said Clinton's campaign is "pressuring" TV stations across the country to stop airing the ad sponsored by the pro-Trump super-PAC.
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The campaign claims it is "directly contradicted by evidence in the public record."
The Clinton campaign contends that the donation was made in a different year, the New York Post report said.
A lawyer for 'Rebuilding America Now', Cleta Mitchell said in the report the advertisement "is well within the scope of important public discourse."
The Trump campaign had published these allegations in a 35-page booklet last month.
Clinton has rebutted these allegations several times in the past.
In an earlier statement, the Trump campaign had cited a New York Times report alleging that as early as 2008, Indian politician Amar Singh had donated between USD 1,000,001 and USD 5,000,000 to the Clinton Foundation.
"Singh visited the US in September 2008 to lobby for a deal allowing India to obtain civilian nuclear technology; then-Senator Clinton assured him democrats would not block the deal," the Trump campaign had alleged.