The agency grilled Manoj for nearly eight hours about alleged misrepresentation done by his companies while applying for coal blocks, CBI sources said.
They said the agency is also scrutinising documents provided by Jayaswals in their defence.
The sources said Manoj was asked about share holdings of his companies which have been named in three cases registered by the agency regarding alleged irregularities in coal block allocation.
While Manoj, owner of Abhijeet Group, has been named by CBI in three cases of alleged irregularities in coal block allocation made to AMR Iron and Steel, JAS Infrastructure and JLD Yavatmal Power, his brother Arvind has been named in a case against AMR Iron and Steel.
The other family members -- Anand and Abhishek -- have been named in cases against JAS Infrastructure and JLD Yavatmal Power.
In the three cases in which the Jayaswals figure, the CBI has slapped allegations of cheating and misrepresentation of facts to grab coveted coal blocks against the companies.
The CBI in its investigation has found that the companies have allegedly given wrong information in their applications claiming they were not in possession of any coal blocks and inflated their net worth to show eligibility for them.