Sorting through sample of sediment from the time period, researchers from the Rensselaer Polytechnic Institute in the US discovered evidence of the strike in the form of microtektites - tiny dark glassy spheres typically formed by extraterrestrial impacts.
"The coincidence of an impact with a major climate change is nothing short of remarkable," said Morgan Schaller, assistant professor at Rensselaer.
Schaller was searching for fossilised remains of Foraminifera, a tiny organism that produces a shell, when he first noticed a microtektite in the sediment he was examining.
Once Schaller noticed the first microtektite, researchers switched to a white sorting tray. At peak abundance, the research team found as many as three microtektites per gram of sediment examined.
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Microtektites are spherical, or tear-drop shaped, and are formed by an impact powerful enough to melt and vaporise the target area, casting molten ejecta into the atmosphere.
Some microtektites from the samples contained "shocked quartz," definitive evidence of their impact origin, and exhibited microcraters or were sintered together, evidence of the speed at which they were travelling as they solidified and hit the ground.
However, the source of the carbon dioxide had not been determined and little is known about exact sequence of events.
One clue can be found in a sudden shift in the ratio of carbon isotopes (atoms containing a number of neutrons unequal to the protons in their nucleus) in certain fossils from the time period.
In particular, Foraminifera, or "forams," produce a shell whose chemistry is representative of atmospheric and ocean carbon isotopes.
The discovery of microtektites was "completely by accident" said Schaller.
Ordinarily, the team passes samples through sieves of various sizes, to isolate samples most likely to contain forams. The tektites, which are smaller than most forams, would have been largely removed in this process.
Once the team made the discovery, they obtained a sample from a fourth site - Medford - where the unit is naturally exposed at the surface. The Medford samples also contained microtektites.