The protest was held outside the Annabhau Sathe auditorium in Satara Road area. However, the play was conducted amid tight security, a senior police official said.
Congress and NCP had earlier warned that they will not allow staging of the play claiming that Nathuram Godse, who had assassinated Mahatma Gandhi, was glorified in it.
"A wrong history is being portrayed through such a play," alleged Santosh Shinde, a leader of Sambhaji Brigade.