It also demanded Fadnavis's resignation.
State Congress spokesperson Sachin Sawant said that participation of IAS and IPS officers was objectionable, as the service rules don't permit them to take part in activities of private organisations.
Citing the All India Service rules, Sawant sought to know if they were forced to take part in the video.
Apart from the chief minister and his wife, the video features Finance Minister Sudhir Mungantiwar, Mumbai municipal commissioner Ajoy Mehta and police commissioner Datta Padsalgikar.
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Sawant also alleged that the sequence featuring Amruta Fadnavis was shot in the core area of the Sanjay Gandhi National Park (SGNP) here, where shooting is not allowed unless a special permission is obtained.
"Tickets are being sold online in the name of River March for a programme on March 4. There should be a detailed inquiry into this," he said.
"The CM should take moral responsibility for violation of norms and we demand that he resign," Sawant said.
River March is not a registered organisation but a people's movement started by morning walkers at SGNP, the CMO said in a statement.
River March started the campaign for cleaning up Mumbai's rivers in 2014, and people such as actor Amitabh Bachchan and activist Anna Hazare supported the cause, it said.
The CMO also said that no tickets are being sold for the organisation's program tomorrow.
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