Thakre alleged that Fadnavis, in a speech made in the Assembly on Monday, made allegations about Thakre' delay in starting a cotton yarn spinning mill in Yavatmal.
Fadnavis had said that Thakre got approval from the government to establish the mill in 1991, and it would have benefited the cotton farmers of the region which is now the worst-hit by the agrarian crisis.
However, Thakre could not set up the mill for over two decades, the CM said.
"He made these allegations in my absence, which is an insult to this house," the Congress leader added.
Fadnavis has denied making the allegations.
Chairman of the Council Ramraje Nimbalkar said he will call for more information and decide whether to admit the motion or not.