The Supreme Court today turned down Navalny's appeal against the Central Election Commission's move, saying that the decision to bar him from the race fully conforms to law.
President Vladimir Putin, whose approval ratings top 80 per cent, is set to easily win a fourth term in the March 18 vote.
Navalny has campaigned for the presidency all year despite an implicit ban on his candidacy due to a fraud conviction seen by many as politically driven.
Election officials formally barred him from the ballot Monday.