The procedure at the University Hospital Centre in Toulouse was executed "in a single go, exclusively with robots," the lead surgeon, Frederico Sallustro, told AFP.
In addition, the kidney was both removed and transplanted through the vagina rather than via an incision, the standard method, the hospital said in a press release.
Valerie Perez, 44, gave one of her kidneys to her 43-year old sister Beatrice Perez on July 9, with both of them in the operating theatre at the same time.
The donor was back at home the day after the surgery, while her sister stayed on in hospital another three days.
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About a hundred people -- notably in India, the US and France -- have benefited from robotic kidney transplants in the last couple of years, according to the hospital.
Sallustro and Doumerc first combined this high-tech method with a transplant to the recipient via the vagina in May. The donor in that case, however, was a man.
With the sisters, the surgical team decided to add a further innovation by completing both parts of the procedure -- removal and insertion -- one immediately after the other.