The letter which went under the hammer at Bonhams on September 21 sold for USD 197,000 - more than twice its estimate of USD 90,000.
The subject of Darwin's religiosity had long been a cause of vehement debate. Darwin himself largely refrained from public comment, probably to respect the feelings of his friends and family.
Darwin's letter is a reply to a young barrister named Francis McDermott. McDermott wrote to Darwin on November 23, 1880, with an unusual request.
"My reason in writing to you therefore is to ask you to give me a Yes or No to the question Do you believe in the New Testament..." McDermott wrote.
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McDermott continues by promising not to publicise Darwin's reply in the "theological papers". Darwin responded the very next day.
"Dear Sir, I am sorry to have to inform you that I do not believe in the Bible as a divine revelation and therefore not in Jesus Christ as the son of God. Yours faithfully Ch Darwin," Darwin wrote.
McDermott was true to his word and this letter was unknown to scholars for over 100 years.