Deadmau5, 34, was infuriated after a video landed online showing the French DJ playing his set while the horse and some bikini-clad dancers wearing Native American headdresses were surrounded by the crowd, reported Aceshowbiz.
"For real... Horses belong on a farm, not in an over-priced nightclub to be subjected to an over-paid DJ," he tweeted.
Deadmau5, whose real name is Joel Thomas Zimmerman, also accused the DJ of animal cruelty and tweeted, "I'm sure the horse really appreciated it."
The video was taken a few weeks ago, during the opening party of Guetta's residency at Pacha Ibiza.