One of the victims, identified as Dikshi, died this morning at a private hospital after she was transferred from Merut District Hospital, they said.
Meanwhile, one Charan Singh died on late Wednesday night.
Uttar Pradesh government announced an ex-gratia of Rs 2 lakh to the next kin of the deceased and Rs 50,000 to the injured ones.
The passengers were all on their way to Haridwar to perform 'mundan' ceremony of a boy when the tractor trolley in which they were traveling was hit by Kotdwar-bound passenger train at an unmanned crossing on Tuesday, police said.