In a letter to the Guardian Council, which oversees elections, Raisi called for "the investigation of certain violations committed before and during the election", according to the Fars news agency.
It said Raisi, a 56-year-old jurist and cleric, had sent hundreds of pages of documents supporting his allegations.
"I cannot keep quiet in the face of the injustice committed against the rights of people," he said in his letter.
The conservative camp had complained of violations before polling had even closed on Friday.
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