The attack is the latest in a spate of political violence that has claimed 75 lives so far in Bangladesh, where the opposition BNP has clamped an indefinite countrywide blockade.
However, no casualty was reported in the incident that occurred near the Iswardi Railway Station at Pabna, police said.
At least nine persons, including three children, were burnt alive when similar attacks occurred in the past two days as opposition activists hurled petrol bombs at a packed bus and a truck.
"No one was hurt. No major damage was done as the train did not catch fire," The Daily Star Online quoted him as saying.
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"The train will head for Dhaka after its engine is changed," he said.
According to a report in bdnews24.Com, Railway police OC Humayun Kabir said three bombs were lobbed at the Dhaka bound train as soon as it reached a Loco Shed after leaving the Iswardi station.
Initial reports did not mention if any person was arrested in this connection.
The incident took place on the first day of a fresh 72-hour spell of general strike, that began today, sponsored by the BNP-led 20-party alliance.
Khaleda Zia's Bangladesh Nationalist Party (BNP) has already enforced a countrywide transport blockade that entered its 30th consecutive day today.