He "relieved" Higher Education Minister K P Anbalagan as the party's Dharmapuri district secretary and bifurcated the Dindigul district unit.
In a statement, Dhinakaran, nephew of jailed party chief V K Sasikala, announced replacing Anbalagan with local leader D K Rajendran.
Anbalagan is the fifth AIADMK minister to be stripped of party posts by Dhinakaran who is involved in a tussle for power with Chief Minister K Palaniswami.
Two days back, Dhinakaran had removed four ministers from party posts in a bid to assert his authority in AIADMK (Amma).
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Today, he also removed Dindigul secretary V Marudaraj and announced that the district was being bifurcated organisation- wise as Dindigul East and West.
Dindigul East would cover four Assembly constituencies while the other would cover three, he said.
Dhinakaran appointed R Thangadurai MLA and K P Nallasamy as secretaries of Dindigul East and West units.
Shaking up the party's medical wing, he relieved S Muthiah and Lok Sabha MP P Venugopal as its president and secretary respectively.
Dhinakaran elevated the medical wing's joint secretary K Kathirkamu, MLA, presently camping at Puducherry with other pro-Dhinakaran legislators, as president of the party's medical wing.
All appointments were being made with the "approval" of AIADMK general secretary Sasikala, Dhinakaran said.