Sources in both AIADMK and DMK confirmed that Rajathi Ammal visited the hospital last night and enquired about the health of Jayalalithaa, who has been under treatment there for infection since September 22.
Her visit comes days after DMK Treasurer M K Stalin, son of Karunanidhi, called on doctors at the hospital.
While DMK sources confirmed that she met Jayalalithaa's aide Sasikala in the hospital and enquired about the health of the Chief Minister, AIADMK sources declined to comment on the meeting.
Her unannounced visit came to light only today.
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Meanwhile, former Tamil Nadu governor K Rosaiah today visited the hospital and enquired about the health of Jayalalitaa.
Rosaiah, whose term as the state governor ended recently, said he prayed the almighty for the speedy recovery of Jayalalithaa.
Ever since Jayalalithaa was hospitalised last month, the hospital has received a steady stream of VVIP visitors including Union Ministers and leaders of political parties besides the all-time presence of AIADMK leaders.