The problem started after Mylapore MLA R Rajalakshmi made some remarks to which both DMDK, the main Opposition party in the Assembly, and DMK objected to even as the party sought to give some explanation.
Agitated DMK MLAs were seen arguing with the Treasury benches.
With the DMK MLAs in no mood to heed Speaker P Dhanapal's repeated pleas to resume their seats, he summoned the Marshals to evict them en masse.
The party had earlier staged a walkout on being denied permission to raise some issue.