Her character Bill Potts' sexuality will be revealed at the beginning of the first new episode when the sci-fi series returns to the BBC on April 15.
Gay and bisexual characters have featured in "Doctor Who" in the past, but this is the first time the main character's companion has come out.
"It shouldn't be a big deal in the 21st Century. It's about time, isn't it? That representation is important, especially on a mainstream show. It's important to say people are gay, people are black - there are also aliens in the world as well so watch out for them," Mackie tells the BBC.
Mackie has replaced Jenna Coleman as the Doctor's new companion, and she admits her life became crazy overnight after she was named the Timelord's sidekick a year ago.
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"My Twitter follower count went from 400-and-something to 16,500 in about two hours, so that was pretty mental (crazy)," she says.
"But it's been really nice; everyone seems to be really excited. It's sort of like being welcomed into a family and all the fans have been really welcoming."
The news of his departure has sparked speculation the next "Doctor Who" will be a woman - another first for the popular TV sci-fi franchise.