Health Department polio vaccination team members Aasiya, her husband Abdul Sattar and Aneela Dawood went to a village in Cholistan region to vaccinate children.
They approached some women who were picking cotton with their children in a field on Sunday.
Jamal Kulyar, who was in the field, instigated the women against the polio team. He called them American spies and accused them of frequently visiting the area to spy on people, Express Tribune reported.
They reported the incident to health officials in Bahawalpur district, who then requested the assistant commissioner to take action.
A complaint was filed at Derawar police station.
Polio vaccination teams have been attacked by militants several times in Pakistan's restive northwest.