Besides, the guild has also sought removal of all expat pilots from the airline.
Jet Airways currently has about 60 expat commanders, who mainly operate its Boeing 737 and ATR fleet.
"NAG has issued a directive to all its members not to fly with the foreign pilots in the airline from May 1 as one of the foreign pilots assaulted a designated examiner during a training session about 10 days ago," a source said.
The guild claims representation of around 1,000 pilots of the total around 1,500 pilots in Jet Airways. The Naresh Goyal-promoted private airline had witnessed a 5-day long strike by NAG in September 2009 over the issue of forming the union.
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In a statement Jet Airways said the airline is an "equal opportunities employer".
"We today employ a multi-cultural workforce...As an Indian airline operating internationally, Jet Airways has always focused on recruiting the brightest talent," it said.