Hundreds of Palestinian travellers were left stranded on both sides of the crossing, the only gateway into the Hamas-ruled Palestinian territory that bypasses Israel, witnesses said.
The measure follows widespread unrest in Egypt on yesterday after a bloody crackdown by security forces on loyalists of ousted Islamist president Mohamed Morsi.
The Egyptian authorities closed the border crossing for several days after the military overthrew Morsi on July 3. It later re-opened for four hours a day.
At least 464 people were killed in violence across Egypt sparked by the crackdown, the health ministry said on Thursday.