They said the 27 include a man and a woman picked up by police yesterday and all have been charged with "debauchery," they said.
The officials spoke on condition of anonymity because they were not authorised to brief the media.
Amnesty International yesterday called for the "immediate" and "unconditional" release of all people arrested since a September 22 concert in Cairo in which an LGBT flag was raised, an incident that sparked the ongoing crackdown.
"The authorities must urgently halt this ruthless crackdown and release all those arrested immediately and unconditionally," said Najia Bounaim, Amnesty's North Africa campaigns director.
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The group that played in the September 22 concert Lebanese indie rock band Mashrou' Leila whose lead singer is openly gay condemned the crackdown in a statement posted on its Facebook page today.
Calling it a witch hunt, it said all those detained must be released and international pressure put on Egyptian authorities to halt the crackdown.