Sisi, who is riding a wave of popularity after ousting Islamist president Mohamed Morsi last July amid massive street protests, is widely expected to win the May 26-27 election.
"With God's will, we will wage a great and victorious battle," Sabbahi, told his supporters after submitting his candidacy to the electoral commission.
Sabbahi has surpassed the 25,000 signatures from citizen supporters required to officially register his candidacy, gathering 31,100 signatures from 17 provinces, according to his campaign team.
Sabbahi was accompanied by scores of supporters, who cheered their candidate and chanted: "Sabbahi is the symbol of freedom!"
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They carried boxes containing the signed forms to be handed over to the electoral committee.
A longtime opposition figure jailed during the rule of strongman Hosni Mubarak and his predecessor Anwar Sadat, Sabbahi came in third in Egypt's first free presidential election in 2012, a year after Mubarak was toppled by an Arab Spring-inspired uprising.
Sisi officially submitted his bid for presidency on Monday, with his lawyer handing over the required documents. His campaign team said they submitted around 200,000 signatures to the electoral committee.
Sisi has dismissed such fears, and his supporters view him as a strong leader who can stabilise the economically- battered country after three years of turmoil.
The electoral commission is to announce the final field of candidates on May 2, and official campaigning starts a day later.