The lights normally illuminating the 324-metre (1,063-foot) tall monument at night were briefly turned off at 8:00 pm (local time) before gradually coming back on.
The symbolic gesture came as thousands of people gathered on the other side of the city in Republique square to hold a second night of vigils in memory of the victims of the worst attack on French soil in decades.
Other events will be held in the French capital in the coming days to mark the outrage over yesterday's attack on Charlie Hebdo, and to assert the right to freedom of expression -- a value France has long cherished and sought to protect.
The Eiffel Tower is one of France's top tourist attractions and the pre-eminent monument in the City of Light.