Researchers studied annual data from more than 5,000 soybean and 5,000 maize farmers in the US from 1998 to 2011, far exceeding previous studies that have been limited to one or two years of data.
"We have repeated observations of the same farmers and can see when they adopted genetically modified seeds and how that changed their use of chemicals," said Federico Ciliberto from University of Virginia in the US.
Since 2008, genetically engineered crops have accounted for more than 80 per cent of maize and soybean crops planted in the US, they said.
Maize seeds are modified with two genes: one kills insects that eat the seed and other allows the seed to tolerate glyphosate, a herbicide commonly used in weed killers.
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Soybeans are modified with just one glyphosate-resistant gene.
Unsurprisingly, maize farmers who used the insect-resistant seeds used significantly less insecticide - about 11.2 per cent less - than farmers who did not use genetically modified maize.
Soybean crops, on the other hand, saw a significant increase in herbicide use, with adopters of genetically modified crops using 28 per cent more herbicides than non-adopters, researchers said.
Ciliberto, who carried the study alongside Edward D Perry of Kansas State University, David A Hennessy of Michigan State University and GianCarlo Moschini of Iowa State University, attributes this increase to the proliferation of glyphosate-resistant weeds.
"In the beginning, there was a reduction in herbicide use, but over time the use of chemicals increased because farmers were having to add new chemicals as weeds developed a resistance to glyphosate," Ciliberto said.
However, the study did find evidence that both maize and soybean farmers increased herbicide use during the last five years of the study, indicating that weed resistance is a growing problem for both groups.
"Evidence suggests that weeds are becoming more resistant and farmers are having to use additional chemicals, and more of them," Ciliberto said.