The asset freezes and travel bans were first introduced in early 2014 as the crisis deepened into outright war between pro-Russian rebels and government forces in eastern Ukraine.
The European Council, representing the 28 member states, said it "prolonged the restrictive measures over actions undermining or threatening the territorial integrity, sovereignty and independence of Ukraine for a further six months until 15 September."
"The assessment of the situation did not justify a change in the sanctions regime," a statement said.
The list previously included Russian Deputy Prime Minister Dmitry Rogozin for his role in the Russian annexation of Crimea in March 2014.
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It also included Arkady Bakhin and Anatoly Antonov, defence and deputy defence ministers, along with tycoon Arkady Rotenberg, a close associate of President Vladimir Putin.
The EU has imposed a whole series of punitive measures over Ukraine where the Minsk ceasefire accords brokered by France and Germany with Kiev, Moscow and the rebels repeatedly fail to hold.
In December, the bloc extended for another six months restrictive measures targeting Russia's defence, financial and energy sectors which were introduced after the July 2014 shooting down of a Malaysia Airlines plane over eastern Ukraine, blamed on the rebels.
Moscow says all the sanctions are ineffective and counter-productive to ending a conflict which has now cost some 10,000 lives.
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