UK-based Oxfam has been left reeling by reports in the Times newspaper that senior staff hired young sex workers in Haiti after the 2010 earthquake which devastated the island.
The charity's chiefs have been summoned by the British government to explain their handling of the scandal amid accusations it covered up the episode.
The European Union said today it took a "zero tolerance" approach to misconduct by NGO partners.
"We expect Oxfam to fully clarify the allegations with maximum transparency as a matter of urgency, and we're ready to review and, if needed, cease funding to any partner who is not living up to the required high ethical standards," spokeswoman Maja Kocijancic told reporters.
Oxfam, which employs around 5,000 staff and has 23,000 more volunteers, has announced new measures to tackle sexual abuse cases, though Chief Executive Mark Goldring has insisted there was no cover-up in 2011.
The charity is accused of failing to warn other aid agencies about the staff involved, which allowed them to get jobs among vulnerable people in other disaster areas.