In a report submitted in July to the then DGP (Prisons) H N Sathyanarayana Rao, Roopa as Deputy Inspector of General (Prisons) had said there was "a talk" that Rs two crore had exchanged hands to give "preferential treatment" for Sasikala and that there were bribery allegations against him also.
Rao had rubbished the charge by Roopa, terming it "absolutely false, baseless and wild", and said he would take legal recourse against his junior.
Puttige Ramesh, advocate for Rao, told PTI that the suit was filed yesterday on various counts in a court here.
The suit is also against a leading Kannada TV channel and a prominent English newspaper.
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He said Roopa did not issue any notice to her superior and directly accused him of wrongdoing, which was a violation of the rules.
She also leaked the information to the media, he said.
The third accusation in the defamation suit isthat she was after publicity.
"We came to know that a movie on Roopa is in the making. In order to gain publicity for the movie, she prepared the defamatory report on my client (Satyanarayana Rao)," he said.
He said Rao had specially appointed a woman officer to keep an eye on Sasikala's activities inside the jail.
The advocate said it was initially planned to file a Rs 50 crore defamation suit, but the requisite deposit amount of Rs30 lakh was not available.
Hence the defamation amount wasbrought down to Rs 20 crore.
Rao was not reachable for his comments.
Meanwhile, Roopa said she has not received any defamation notice.
"I have not received any defamation notice. If I receive, I will answer in the court. As it is, there is no defamation made out-- that is the first thing," she said.
Roopa said it was for Rao to explain why these irregularities happened under his nose.
The two officers were locked in a public spat over the bribery charge, which led to the state government asking Rao to go on leave and Roopa being transferred.
Sasikala is lodged in prison here since her conviction in February in the disproportionate assets case, along with her two relatives V N Sudhakaran and Elavarasi, all serving a four-year jail term.